Question

02 The rate of migration of a protein in an SDS-polyacrylamide gel is not influenced by a. size of the protein b. charge of the protein c. pore size of the gel d. strength of the electric field ​

Answer 1

Answer:

SDS-PAGE separates proteins primarily by mass because the ionic detergent SDS denatures and binds to proteins to make them uniformly negatively charged. Thus, when a current is applied, all SDS-bound proteins in a sample will migrate through the gel toward the positively charged electrode

Explanation:

Answer 2

Answer:

The rate of migration of a protein in an SDS-polyacrylamide gel is not influenced by charge of the protein

Explanation:

Protein separation is a function of an analytical technique called SDS polyacrylamide gel. SDS pages are a form of electrophoresis method that classifies compounds as per their size.

The pace at which a protein migrates through an SDS polyacrylamide gel is not affected by the protein's charge. All proteins' charges are neutralized by the addition of SDS to the gel.

Even though the ionic detergents SDS hydrolyzes and binds to molecules to make them evenly negatively charged, SDS-PAGE predominantly separates proteins by mass. All SDS-bound molecules in a sample will therefore move across the gel and towards the positive charged electrode whenever a current is delivered.

To know more about SDS polyacrylamide gel, visit:

https://brainly.in/question/6882765

To know more about negative charge, visit:

https://brainly.in/question/44341047

#SPJ3