Question

(1)
214 Name the substance you could add to the enzyme substrate mixture in order to
stop enzyme activity at 35 degrees?​

Answer 1

Answer:

acid or base can be added

Answer 2

In enzyme analysis, the simple way to stop an enzyme reaction is to add trichloroacetic acid (TCA).

Explanation:

• Enzyme activity can be affected by various factors such as temperature, pH and concentration.

• There are three basic types of enzyme inhibition: competitive, non-competitive and non-competitive.

• In competitive inhibition, an inhibitor molecule is so similar to a substrate that it can bind to the active site of the enzyme to prevent it from binding to the substrate.
• Competitive inhibitors compete with substrates for the same binding site on the enzyme.
• Competitive inhibitors, as the name suggests, compete with the substrate to bind the enzyme at the same time.
• The inhibitor has an affinity for the active site of an enzyme where the substrate also binds.