Physics, asked by santhoshbaabu17807, 2 months ago

1. Describe various steps involved in tissue culture.​

Answers

Answered by C0R0NA
1

Describe the various steps involved in tissue culture.

1) Selection of plant:

  • The plant which has to be cultured in vitro is selected.

2) Isolation of explant:

  • Explant is any part of plant excised out for tissue culture.

3) Sterilization of explant:

  • Explant is surface sterilized so as to avoid contamination.

4) Inoculation of explant:

  • The explant is inoculated on the nutrient medium.

5) Incubation:

  • After inoculation, the cultures are incubated so as to provide proper conditions for their growth & regeneration like temperature, moisture etc.

6) Initiation of callus:

  • Callus is a mass of undifferentiated cells formed by the dedifferentiation of plant cells or the explant.
  • Callus further regenerates to form roots & shoots & eventually the complete plant.

7) Sub-culturing:

  • The cultured cells or tissues are transferred regularly to new nutrient medium called subculturing.

8) Regeneration:

  • Regeneration is the formation of organized structures like roots, shoots, flower buds etc from the cultured cells

9) Hardening:

  • The plantlets are removed from the cultures and prepared for soil transfer & this transfer to fields is known as hardening.
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