Question

1.different between ziehl cold stain technique AND ziehl hot stain technique​

Answer 1

Answer:

E

Explanation:

Answer 2

Answer:

ziehl cold stain technique:-

The Ziehl Neelsen (ZN) method of staining acid fast bacilli has been in vogue for more than hundred years. In the ZN method [1], the basic fuchsin phenol dye is used hot there by melting the unsaponifiable waxy substance on the surface of the cell wall. However, because of disadvantages like controlled heating and non-availability of spirit for lamps there have been attempts to develop a cold staining method for tubercle bacilli.

ziehl hot stain technique:-

The Ziehl-Neelsen stain uses basic fuchsin and phenol compounds to stain the cell wall of Mycobacterium species.

Mycobacterium does not bind readily to simple stains and therefore the use of heat along with carbol-fuschin and phenol allows penetration through the bacterial cell wall for visualization.

Mycobacterium cell wall contains high lipid content made up of mycolic acid on its cell wall making it waxy, hydrophobic, and impermeable. These are ß-hydroxycarboxylic acids made up of 90 carbon atoms that define the acid-fastness of the bacteria.

Use of Carbol-fuschin which is basic strongly binds to the negative components of the bacteria which include the mycolic acid and the lipid cell wall. addition of acid alcohol along with the application of heat forms a strong complex that can not be easily washed off with solvents.

The acid-fast bacilli take up the red color of the primary dye, carbol-fuschin.

While non-acid-fast bacteria easily decolorize on the addition of the acid-alcohol and take up the counterstain dye of methylene blue and appear blue

This technique has been used in the identification of Mycobacterium tuberculosis and Mycobacterium leprae.