Question

1) Explain synthesis of genetically engineered human insulin.
2)How is Rosie considered different from a normal cow?
3)What are cry proteins ?Name an organism that produces them.How has man exploited the protein?
4)Expand GEAC n ELISA
5) Mention the steps taken during gene therapy to treat ADA deficient patient.
6)Name the nematode that damages the roots of tobacco plants.How is transgenic tobacco plant made resistant to nematode using bacteriophage.?
7)What is GMI?List three advantages
8)What is biopiracy?

Answer 1

• A small section is then cut out of the circular plasmid by restriction enzymes, 'molecular scissors'. The gene for human insulin is inserted into the gap in the plasmid. ... The more the cells divide, the more insulin is produced.
• Rosie was a transgenic cow. It was considered different from a normal cow as it produced human protein enriched milk. The milk contained 'human alpha-lactalbumin' and was nutritionally a more balanced product for human babies than the normal cow milk.
• Cry proteins are encoded by cry genes. These proteins are toxins, which are produced by Bacillus thuringiensis bacteria. This bacterium contains these proteins in their inactive from. When the inactive toxin protein is ingested by the insect, it gets activated by the alkaline pH of the gut.
• ELISA or EIA is an enzyme-linked immunosorbent assay. It is a test that detects and measures antibodies in our blood. This test can be used to determine if we have antibodies related to certain infectious conditions. It is used to diagnose HIV, which causes AIDS.Genome Engineering Action Committee
• the European Commission granted market approval to GlaxoSmithKline (GSK) for ex vivo hematopoietic stem cell (HSC) gene therapy for the treatment of adenosine deaminase (ADA)‐deficient severe combined immunodeficiency (SCID), a very rare congenital disorder of the immune system.
• Root knot nematode of tobacco is caused by Meloidogyne nematodes, including Meloidogyne incognita (southern root knot nematode), Meloidogyne javanica (Javanese root knot nematode), Meloidogyne hapla (northern root knot nematode), Meloidogyne arenaria (peanut root knot nematode), and the more recently introduced