Question

1.
In what order will the following proteins be eluted from a CM-cellulose ion exchange resin (cution
exchange column) as the pH of the clution buſſer is ramped from a pH of 3 to a pH of 12-fibrinogen
(pl=58). hemoglobin (pl=7.1. lysozyme (pl=110), and ovalbumin (pl=4.6).
Given the following 5 peptides:
Chargent p16.0 Molecular Wright
Peptide A
-2
670 amu
Peptide B
1085 amu
Peptide
0
$45 amu
Peptide D
-5
1120 amo
Peptide E
$00 amu
pl Value
34
8.6
6.8
65
a) Show the separation of peptides by polyacrylamide gel electrophoresis with SDS (detergent)
b) Show the separation of peptides by PAGE.
c) 'Show the separation of the peptides by isoelectric focussing on a gel with a pH gradient from 3 to 9​

Answer 1

Answer:

first according to the question