Question

1) In which bioassay technique chlorophyll retention test is used?
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Answer 1

Answer:

answer is

Qualified cell - Based Theory

Explanation:

A bioassay is an analytical technique to measure a substance's quantity or strength by observing how it affects living creatures like plants or animals (in vivo), as well as living cells and tissues (in vitro).A bioassay can be direct or indirect, quantal or quantitative.  The test is quantal if the observed reaction is binary; otherwise, it is quantitative. A bioassay can be used to determine a mixture's grade or to identify biological threats. A bioassay is frequently used to check on water purity, wastewater emissions, and environmental effects.

The safety and environmental effect of new facilities and innovations are also evaluated using this method.

Principle

A biochemical test to determine the strength of a sample substance is called a bioassay. Typically, this strength can only be evaluated in comparison to a reference substance. [3] [1] In a normal bioassay, a person is exposed to a stimulant (for example, medications) (ex. animals, tissues, plants). This causes and measures the subject's related reaction, such as mortality.

History

As early as the late 19th century, when German surgeon Paul Ehrlich established the groundwork for bioassays, a bioassay was used for the first time.

He proposed the idea of standardization using living things' responses.

His experiment on the diphtheria poison was the first to be acknowledged [6].

He was able to determine through the use of bioassay that giving animals a steadily rising dosage of diphtheria promoted the production of antiserum.

The experiment known as "the canary in the coal mine" is an effective illustration of a bioassay. Miners would bring methane-sensitive canaries into coal mines to provide early notice of hazardous amounts of methane in the air. The workers would evacuate the region as soon as possible if the canary perished as a result of a methane buildup.

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