1. Mention the Two major kinds of chlorophyll pigments present in thyikoid
membrane?
Answers
Answer:
ᴄᴀʀᴏᴛᴇɴᴏɪᴅs ʀᴇsɪᴅᴇ ᴡɪᴛʜ ᴛʜᴇ ᴛᴡᴏ ᴛʏᴘᴇs ᴏғ ᴄʜʟᴏʀᴏᴘʜʏʟʟs ɪɴ ᴛʜᴇ ᴛʜʏʟᴀᴋᴏɪᴅ ᴍᴇᴍʙʀᴀɴᴇ. ᴄᴀʀᴏᴛᴇɴᴏɪᴅs ᴀʀᴇ ɪᴍᴘᴏʀᴛᴀɴᴛ ʙᴇᴄᴀᴜsᴇ ᴛʜᴇʏ ᴄᴀɴ ᴀʙsᴏʀʙ ᴄᴇʀᴛᴀɪɴ ᴡᴀᴠᴇʟᴇɴɢᴛʜs ᴏғ ʟɪɢʜᴛ ᴛʜᴀᴛ ᴄʜʟᴏʀᴏᴘʜʏʟʟs ᴄᴀɴɴᴏᴛ. ᴛʜᴇsᴇ ᴀʀᴇ ᴛʜᴇ sᴛʀᴜᴄᴛᴜʀᴇs ᴛʜᴀᴛ ᴄᴀᴜsᴇ ʟᴇᴀᴠᴇs ᴛᴏ ᴀᴘᴘᴇᴀʀ ᴏʀᴀɴɢᴇ ᴀɴᴅ ʏᴇʟʟᴏᴡ.
Explanation:
Thylakoid membranes from spinach were fragmented mechanically and separated into vesicles originating from grana and stroma-exposed lamellae (Andreasson et al. (1988) Biochim Biophys Acta 936: 339–350). The grana vesicles were further fragmented and separated into smaller vesicles originating from different parts of the grana (Svensson and Albertsson (1989) Photosynth Res 20: 249–259). All vesicles so obtained were analyzed with respect to chlorophyll and carotenoid composition by reverse phase HPLC. For all fractions the following relations (mole/mole) were found: 1 carotenoid per 4 chlorophyll (a+b), 2 lutein per 5 chlorophyll b and 5 violaxanthin per 100 chlorophyll (a + b). The contents of lutein and neoxanthin were each linearly related to chlorophyll b and β-carotene was linearly related to chlorophyll a.