1. Preparation of hemin and hemochromogen crystals
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Explanation:
A small amount of dry blood is taken on a glass slide and crushed to a fine powder with the help of the fused end of a glass rod or with a needle. One crystal of common salt (NaCI) is added to it, which is also crushed to powder. The two are thoroughly mixed and two drops of glacial acetic acid added to it.
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Explanation:
Haem is made up of four pyrrole rings and a central iron ion in ferrous state. The haemin crystals are prepared by heating of the blood with Nippe's reagent. This ruptures the RBCs and the haemoglobin is released. Also, in this process the ferrous form of iron is converted to ferric form.
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