Question

1. The extinction coefficient for a particular protein is 0.685 Lmol-1cm-1. Which value is closest to the protein concentration if the absorbance is 7 and the path length is 1 cm? A) 0.001 M B) 0.1 M C) 1 M D) 10 M 2. A neurogenetic researcher is attempting to confirm the presence of a particular neurotrophic factor (called ABNF) in a cell extract. She runs a sample through an SDS-PAGE gel and observes a single dark band. Which of the below statements may be true? 1. Only ABNF is present in the sample. II. Only a contaminant protein is present in the sample. III. The sample could contain many distinct samples if all had equal or nearly equal masses. A) I only B) II only C) I and II D) I, II, and III​

Answer 1

Explanation:

The DNAJC protein family is a subclass of heat shock proteins that has attracted recent attention due to the identification of mutations that are linked with parkinsonism, a feature of Parkinson's disease and other neurological disorders. In this review, we discuss the current genetic and functional evidence of the association of these DNAJC proteins with disease and how mutations in these proteins may contribute to disease pathogenesis. Although DNAJC6 (Auxilin), DNAJC12, and DNAJC5 (CSPα) exhibit strong genetic association with disease, DNAJC26 (GAK), DNAJC13 (RME-8), and DNAJC10 (Erdj5) require additional evidence to definitively link reported variants to parkinsonism. Remarkably, multiple DNAJC proteins (Auxilin, GAK, RME-8, CSPα) functionally converge on pathways of synaptic trafficking and clathrin dynamics, highlighting an important role of those pathways in the pathogenesis of parkinsonism. Further research is required to define the mechanisms through which these mutations contribute to disease etiology.