Question

1) what are molecular scissors.give example? <br /><br />explain the role in recombinant dna technology with the help of example? <br />.<br />2) characteristics of cloning vector. describe?<br /><br />why dna cannot pass through cell membrane.explain?<br /><br />how bacterial cell is made competent to take up recombinant dna from the medium? <br />.

Answer 1

• Molecular scissors are basically restriction enzymes. These enzymes make one incision on each of the two strands of the DNA.


• Recombinant DNA Technology helps in the manufacturing of the novel enzymes which are needed in food processing.


• Cloning vector is a small piece of DNA which is taken from a virus, or a plasmid, or the cell of a higher organism.

• Since the DNA is hydrophilic, it cannot pass through the cell membrane.