Question

1) Which of the following parameters is a measure of the affinity of the enzyme for its
substrate in kinetic analysis?
A) Substrate Concentration
B) 1/2 Vmax
C) Vmax
D) KM
E) Reaction Rate

2) Which of the following is not a method for determining the structure of an enzyme?
A) Electron Paramagnetic Resonance
B) High Pressure Liquid Chromatography
C) Cryo-Electron Microscopy
D) X-ray Crystallography
E) Nuclear Magnetic Resonance

3) In which of the following situations might it be desirable to produce an enzyme in laboratory strain bacteria, rather than the original host organism
A) When the original host is a
plant with slow growth rate
B) When the original host only
produces low levels of the enzyme
C) When the enzyme needs to be
tagged and purified for study
D) All of the above
E) None of the above

4) Which of the following statements about rational design is most accurate?
A) It requires intense screening but not extra strutural / mechanistic / molecular modelling data
B) None of the above
C) It requires extra strutural / mechanistic / molecular modelling data but not intense screening
D) It requires intense screening and extra strutural / mechanistic / molecular modelling data
E) It requires neither strutural / mechanistic / molecular modelling data nor intense screening

Answer 1

Explanation:

rate

B) When the original host only

produces low levels of the enzyme

C) When the enzyme needs to be

tagged and purified for study

D) All of the above

E) None of the above

4)

Answer 2

Answer:

1 - KM

2- High Pressure Liquid Chromatography

3- All of the above

4-It requires extra strutural / mechanistic / molecular modelling data but not intense screening

Explanation:100% confirm