1.why must a cell be divided complaint in biotechnology experiment how does biotechnology experiments how does calcium ion help in doing so ?
2. State the role of "biolistic gun" in biotechnology experiments.
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1. DNA being a hydrophilic molecule, can not cross through cell membranes, hence the cell should be made competent to accept the DNA molecules as competency is the ability of a cell to take a foreign DNA.
Calcium ion helps in increasing deposits in cell valve which enables the cell to take up the Recombinant DNA.
2. To introduce alien DNA into hot sell suitable for plant cell and bombarded with high velocity microparticles of gold or tungsten coated with DNA molecules known as biolistic or gene gun which play important role in biotechnology experiments.
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