10:17 AM ORBI
Q9 The average molecular weight(Mr) of 1 point
an amino acid residue in a protein is
about
O
130
O O
120
O
110
O
118
Answers
Answered by
0
Metric Prefixes
Prefix Symbol Factor
kilo k 103
centi c 10-2
milli m 10-3
micro µ 10-6
nano n 10-9
pico p 10-12
femto f 10-15
atto a 10-18
zepto z 10-21
Spectrophotometric Conversions
1 A260 unit of double-stranded DNA=50 µg/ml
1 A260 unit of single-stranded DNA=33 µg/ml
1 A260 unit of single-stranded RNA=40 µg/ml
DNA Molar Conversions
1 µg of 1,000 bp DNA
≈
1.52 pmol(3.03 pmol of ends)
1 µg of pBR322 DNA
≈
0.36 pmol DNA
1 pmol of 1,000 bp DNA
≈
0.66 µg
1 pmol of pBR322 DNA
≈
2.8 µg
pBR322DNA:(4,361 bp)
Formulas for DNA Molar Conversions
For dsDNA
To convert pmol to µg:
pmol × N × 660 pg/pmol × 1 µg/106 pg = µg
To convert µg to pmol:
µg × 106 pg/1 µg × pmol/660 pg × 1/N = pmol
For ssDNA
Prefix Symbol Factor
kilo k 103
centi c 10-2
milli m 10-3
micro µ 10-6
nano n 10-9
pico p 10-12
femto f 10-15
atto a 10-18
zepto z 10-21
Spectrophotometric Conversions
1 A260 unit of double-stranded DNA=50 µg/ml
1 A260 unit of single-stranded DNA=33 µg/ml
1 A260 unit of single-stranded RNA=40 µg/ml
DNA Molar Conversions
1 µg of 1,000 bp DNA
≈
1.52 pmol(3.03 pmol of ends)
1 µg of pBR322 DNA
≈
0.36 pmol DNA
1 pmol of 1,000 bp DNA
≈
0.66 µg
1 pmol of pBR322 DNA
≈
2.8 µg
pBR322DNA:(4,361 bp)
Formulas for DNA Molar Conversions
For dsDNA
To convert pmol to µg:
pmol × N × 660 pg/pmol × 1 µg/106 pg = µg
To convert µg to pmol:
µg × 106 pg/1 µg × pmol/660 pg × 1/N = pmol
For ssDNA
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