10) The small amount of energy available in methanogenic processes forces the microorganisms involved in very efficient cooperation. In many cases, 5 points
neither partner can operate without the other, that is, together, they may exhibit a metabolic activity that neither one could accomplish on its own Such cooperations are
called syntrophic relationships. A typical example is the Methanobacillus meliansky culture, which is a co-culture of two partner organisms, strain S and strain Mo H. The
two strains cooperate in the conversion of ethanol to acetate and methane by interspecies hydrogen transfer, as follows:
Strain S: CH3CH2OH(aq) + H2O(aq)
Strain Mo H.: 4H2 (9) + CO2(g)
CH3COO (aq) + H+ (aq) + 2H2 (9) (1)
CH4 (g) + 2H20 (aq)
(2)
The critical point in this syntrophic cooperation is that the reaction that Strain Sis catalyzing (Eq. 1) is only exergonic (negative 4Gº ) if the H2 partial pressure (or the
corresponding H2 concentration) in the aqueous phase is low enough. Hence, strain M.0.H. must remove hydrogen efficiently from the solution
Calculate the maximum PH2 (H2 partial pressure) at which strain S can just gain some energy from catalyzing reaction Eq. 1 at pH 7 and 25° C, by assuming that both
ethanol and acetate are present at a concentration of 10-6
O 0.28 bar
1.2 bar
O 0.14 bar
O 1.4 bar
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