17) Explain what is HPLC?
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High-performance liquid chromatography (HPLC) is an analytical technique to separate, identify, and quantify components in a mixture. It is the single biggest chromatography technique essential to most laboratories worldwide.
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HPLC stands for High Performance Liquid Chromatography; it is an equipment and method which is used to separate bio-molecules according to their size. In this technique, a pressurized liquid along with sample is allowed to pαss through a column filled with sorbent. Moreover, the bio-molecules will be separated according to the interaction with the sorbent.
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