Question

18. How will you avoid the growing of microbes in nutrient medium during
culture process? What are the techniques used to remove the microbes?​

Answer 1

Answer:

the microbial growth in cultural medium can be overcome by autoclaving the medium at plant tissue culture for 15 to 30 minutes .

Techniques used to remove the microbes are as follow :-

1) sterilizing using UV radiation.

2) chemical sterilization using chemicals.

3) alcoholic sterilization using ethanol.

4)autoclaving

5)filtration . etc...