184
June 2014
036. To test whether bacteria with enhanced toluene degradative abilities could be created for cold environment, a TOL
(Toluene-degrading) plasmid from a mesophilic bacterial strain was transferred by conjugation into a facultative
psychrophile. The psychrophile was able to degrade salicylate (SAL) but not toluene. The recombinant strain
carried the introduced TOL plasmid and its own SAL plasmid. The results are as follows:
Optimum growth in presence of
Х
Y
A B
A B C
A
в с
Temp(°C)
37°C
-
0 1
+
0 1 1 +
25°C
-
+
10°C
+
0°C
+
+
+
Identify A, B, C, X and Y:
a. A-mesophile, B-psychrophile, C-transformant, X-Toluene, Y-Salicylate.
b. A-mesophile, B-psychrophile, C-transformant, X-Salicylate, Y-Toluene.
C. A-psychrophile, B-transformant, C-mesophile, X-Salicylate, Y-Toluene.
d. A-transformant, B-psychrophile, C-mesophile, X-Toluene, Y-Salicylate.
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