Question

19 Following method of ELISA is used to measure the concentration of Ab in sample
A). Direct
B). Indirect
C). Dot blot
D). Sandwitch
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Answer 1

Answer:

Options A is right answer

Explanation:

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Answer 2

Answer:

B). Indirect

Explanation:

• Inhibition ELISA is a sort of indirect ELISA that is primarily used to identify interference in an anticipated signal output in order to calculate the amount of an antibody in a sample.
• To measure immunological responses, indirect ELISA is used to identify antibodies in a sample. The targeting antibody is first immobilized on the plate using a particular capture antigen, and this antigen-antibody combination is then identified using a different antibody.
• A two-step ELISA known as an indirect ELISA includes the binding of a primary antibody and a secondary antibody that has been labeled. After the main antibody has been exposed to the antigen, the secondary antibody is then exposed.
• Sensitivity: Because each primary antibody has numerous epitopes, more than one tagged antibody can bind the primary antibody. No label interacts with the locations on the main antibody, resulting in maximum immunoreactivity. Cost-effective since fewer tagged antibodies are needed.
• Either a qualitative or quantitative ELISA procedure might be used. Qualitative findings provide a sample straightforward yes/no answer.

So Indirect ELISA refers to a two-step ELISA which involves two binding processes of primary antibody and labeled secondary antibody