2,3'-dideoxyribonucleotide
4.
A DNA sequencing reaction was performed with the fragment 5'-XXXXGCGATCGYYYY-3' as the template,
dideoxy GTP, all the four dNTPs, and the required primers and enzyme. XXXX and YYYY in the given DNA
fragment represent primer binding sites. The set of fragments obtained during the reaction will be (the
primers are not shown in the amplified fragments)
a. 5'-CGATCGC-3' only
b. 5-CG-3, 5-CGCTAG-3', 5'-CGCTAGC-3'
c. 5'-CG-3, 5'-CGATCG-3, 5'-CGATCGC-3'
d. 5-G-3, 5'-GCG-3, 5'-GCGATCG-3'
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Answer:
c. 5'-CG-3, 5'-CGATCG-3, 5'-CGATCGC-3 is the right answer...
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