. . . 2.5 Nakula investigated the effect of heat on amylase. Amylase is an enzyme that makes starch molecules break down into sugar molecules. Nakula put some amylase solution into two boiling tubes, ра P and Q He boiled the solution in tube P. He did not heat tube Q He waited until the solution in tube P had cooled down to room temperature He added equal volumes of starch solution to tube P and tube Q boiled amylase amylase After 10 minutes, be tested both tubes for sugar. and starch and starch Nakula found that there was sugar in tube Q, but not in tube P. a What was the variable that Nakula changed in his experiment? [0 b State two variables that Nakula kept constant in his experiment. [2] с Nakula wrote this conclusion to his experiment: "My results show that boiling destroys amylase'. Explain how Nakula's results support his conclusion, [3] . 2 Food and digestion 31
Answers
a) The variable which is changed: TEMPERATURE
b) The two variable which is kept constant is concentration of starch solution and incubation time of the reaction (10mins)
c) Yes, amylase when boiled, the activity of that enzyme is lost we can conclude that by seeing that the starch was not converted to sugar.
Explanation:
a) The temperature was kept variable because only one the test tube which is the P test tube was boiled and the Q test tube was kept in room temperature.
b) The amount of starch solution added to the test tube is same. And the time period for the reaction was 10mins. Both the test tube was checked for the presence of sugar after 10 mins.
c) When a particular enzyme is subjected to heat or temperature change , the enzyme undergoes denaturation in other words heat destroys the enzymes. Due to which the enzyme is no longer able to convert starch to sugar units. Thus in the P test tube only starch was present and not sugar.
Other links to refer from:
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https://brainly.com/question/20226444
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