Question

2 explain primary structure of protein with example

Answer 1

The primary structure of proteins is the sequence of amino acids in a polypeptide chain, linked through peptide bonds, that form the covalent backbone of the proteins. The sequence of amino acids is read from the N-terminal amino acid to the C-terminal amino acid.

 

It is important to realize that two proteins that have the same amino acid composition, can have very different primary structure. These two peptides, for example (a peptide is a short amino acid chain formed by a few amino acids), are formed by the same amino acids, but their primary structure is different, since the sequence is different.

 

H2N-Glu-Ala-Val-Ser-Leu-Ala-Lys-Cys-COOH

H2N-Ala-Glu-Val-Ser-Ala-Leu-Lys-Cys-COOH

 

The primary structure determines the three-dimensional structure of the protein, which in turns determines its biological function. Alteration in normal primary structure of proteins can produce catastrophic results.