Question

(2) The column in HPLC is made of..... or .... to withstand high temperature,
(a) heavy glass, stainless steel
(b) fused silica, polymeric material
(c) thick glass, Copper metal
(d) aluminium metal, stainless steel​

Answer 1

Answer:

heay glass . stainless steel

Answer 2

(a) heavy glass, stainless steel.

(2) The column in HPLC is made of heavy glass or stainless steel to withstand high temperature.

• In HPLC, the analytical column is typically protected and given extra time to last by a guard column.
• Particulate debris, pollutants, and molecules that bond to the column permanently are removed by the guard column.
• The stationary phase of the guard column is comparable to that of the analytical column.
• The most popular material for HPLC columns is stainless steel, but they can also be created from thick glass, polymers like polyetherethelketone, a mixture of stainless steel and glass, or a combination of stainless steel and polymers.
• Analytical HPLC columns typically range in length from 3 to 25 cm and have a 1 to 5 mm diameter.
• Unlike GC columns, the columns are often straight. The columns' packing particles typically have a diameter of 3 to 5 m.
• The efficiency of liquid chromatographic columns will rise as the diameter of the tightly packed particles inside the column decreases.

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