Question

2 types of nucleoprotiens and its functions

Answer 1

The two types of nucleoproteins were studied in resting cells and during mitosis by the May-Grunwald and Giemsa method, following fixation in absolute methanol. Normal cells, cells grownin vitroand leukaemic cells of a transmissable acute leukaemia in ahybrid FAK1mouse strain were used for this investigation. It was found that deoxyribonucleoproteins stained purple-red, and ribonucleoproteins blue. This was also shown by stainingthe isolated nucleoproteins.Deoxyribonucleoproteins occur in the chromosomes of the resting nucleus and in the chromosomes throughout all phases of mitosis. Ribonucleoproteins were found in the cytoplasm of all undifferentiated cells, in the nucleoli of all cells investigated and in the chromosomes from the end of prophase, through meta- and anaphase, up to early telophase. During anaphase ribonucleoprotein appears to be shed from the chromosomes into the cytoplasm between the two groups of anaphase chromosomes.Ribonuclease digests away all ribonucleoprotein. Deoxyribonuclease, in the presence of proteolytic inhibitors, removes from the cells deoxyribonucleoproteins. Neither enzyme acted when specific inhibitors were added. When the cells were treated with either lanthanum or beryllium ions, the resulting nucleoprotein complexes were not attacked by their respective nucleases in the times sufficient for the digestion of the nucleoproteins in untreated cells.