Question

2. What is the necessity of adding Leishman stain first and then diluting it?​

Answer 1

Answer:

The stain must be diluted for use with Phosphate buffer to pH 6.8 or 7.2, depending on the specific technique used. The pH 6.8 is preferred when the morphology of blood cells is to be examined and pH 7.2 is good for parasitic studies.