Question

22. What is DNA cloning? Describe the use of restriction endonucleage​

Answer 1

This is the answer of your question.

Answer 2

DNA  cloning.

Explanation:

• DNA  cloning is a technique that is used for making identical copies of a piece of DNA , or a gene .
• There are following steps in DNA cloning -

• In cloning of a piece of DNA the target gene is inserted into a vector called plasmid.
• This vector is introduced into the bacterial cell by transformation and bacteria carrying plasmid are selected using antibiotics .
• Bacteria with the suitable plasmid is  allowed to divide to make more DNA , or expression of gene for desired protein.
• Restriction endonuclease acts as molecular scissors and help in cutting DNA at specific locations or site.