25. Two healthy green plants were placed in the dark for24 hours. They were then set-up as shown in the figure and left for 4 hours. Then a leaf was taken from each plant and the chlorophyll was removed from the leaves.(a) How is the chlorophyll removed ?(b) What is the next and final step in this experiment?(c) What would be the result of the final step ?(d) Why is it necessary to grease the glass sheet?(e) What hypothesis is being tested in this experiment?(f) Which of the two plants would serve as the control?
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a) Chlorophyll is removed by completely immersing the leaf inside alcohol in a test tube, then placing the test tube over a water bath and heating the water. After some time the leaf would decolorize, at last wash the leaf under tap water. And the chlorophyll is now removed.
b) Last test is having the Iodine test
c) The parts of leaf having starch will turn blue-black
d) It necessary to grease the glass sheet To avoid folding of leaf, and having more accurate results.
e) The need of CO₂ of photosynthesis is being tested.
f) Plants with single colored and large leaf would serve the purpose like Jasmine leafs, Fiddle Leaf Fig, etc.
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