Question

26. A student carried out paper chromatography on a mixture of amino
acids
The student sprayed the dried chromatogram with a locating agent.
What is the function of the locating agent?
A to dissolve the amino acids
B. to form colored spots with the amino acids
C. to preserve the amino acids
D. to shop the amino acids reactina​

Answer 1

Answer:

Function of locating agent is :-

• to form colored spots with the amino acids.

Answer 2

The function of the locating agent is to form coloured spots by reacting with the amino acids.

• Paper chromatography is a convenient and easy technique that involves the separation of amino acids. Such a chromatography is a kind of partition chromatography.
• Amino acids involved in the technique of paper chromatography are colourless compounds.
• So, on spraying the locating agent which is a solution of ninhydrin on the dried chromatography paper then the solution of ninhydrin reacts with amino acids to form coloured spots by producing a purple compound.

Hence, Coloured spots are formed by the locating agent when they react with amino acids.