Question

26. For the better resolution of minute 1 poin
protein bands in SDS-PAGE, which of
the following staining method is
advised?
O A. CBB Staining
B. Silver staining
Ο Ο Ο Ο
C. Avidin staining
O D. All of these​

Answer 1

Answer:

Obtaining a specimen involves the collection of tissue or fluids for laboratory analysis or near-patient testing, and may be the first step in determining a diagnosis and treatment (Dougherty and Lister, 2015). The procedure used to collect a specimen must minimise the risk of introducing error and protect the health and safety of both the patient and the staff who handle the sample. This article, the first in a four-part series, explores the general principles of specimen collection and describes how to collect a midstream specimen of urine (MSU).

Specimens are an important part of a holistic assessment and can help to:

Build a clinical picture of the patient;

Confirm a diagnosis;

Inform a treatment plan.

Explanation:

Answer 2

The Answer is:

Option (B) Silver Staining

Silver binds to the molecular side chains of said amino acid residues, particularly the carboxyl & sulfhydryl groups of the amino acids, making silver staining a potent method for protein analysis in gels. It was first used for polyacrylamide gel electrophoresis in 1972 and was later modified for protein separation.

• The reducing of silver particles by formaldehyde into minute grains of elemental silver facilitates their detection and is facilitated by a nucleation sites in proteins.
• The tertiary structure of such proteins is unaltered by the treatment.
• Due to its high sensitivity, which is often two orders of magnitude better than that of the Ponceau and Coomassie staining procedures and might even detect as low as 2 ng protein, it has a wide range of uses.
• Based on the silver impregnation, there are two major methods for silver staining are alkaline and acidic.
• The silver nitrate diamine complex is used in the alkaline technique in an alkaline environment.
• Better peptide coverage is possible with increased sensitivity, less interference, and lowest mass spectrometry influence thanks to the silver staining.

SPJ3