Question

29. Most of the bacteriological media are
sterilized by​

Answer 1

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Answer,

• The sterilization of media by ethylene oxide is accompanied by a rise in pH.

• Fluids which have a relatively high initial pH may require adjustment with sterile acid to return them to the optimal pH for bacterial growth.

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Answer 2

Answer:

Agar-free media will usually dissolve on gentle agitation. Media containing agar should be heated to dissolve the agar before autoclaving. Bring the medium to the boil without scorching or burning. Most culture media will require final sterilization in an autoclave at 121°C for 20 minutes.