2Q. Why are two antibiotics used to screen for incorporation of a plasmid? Why do some of
the transfected bacteria grow on plates containing ampicillin?
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Ampicillin is an antibiotic and works by preventing E. coli from constructing cell walls, thereby killing the bacteria. When the ampicillin-resistance gene is present, it directs the production of an enzyme that blocks the action of the ampicillin, and the bacteria are able to survive.
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