3. Determine the matrices A and B when
A + 2B = 1 2 6 -3
and 2A - B = 2 -1 2 -1
Answers
Step-by-step explanation:
EGCG inhibits influenza A infection at the initial stage of viral replication. (A) Single kinetic growth curves of influenza A virus on MDCK cells. MDCK cells were infected with 100 TCID50 influenza A virus for 2 h at 4 ºC. After removing unbound virus, cells were replaced with serum-free medium. Supernatants and cell lysates were collected at the indicated times (at 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, and 30 h post-adsorption). Viral RNAs were extracted and quantified by qPCR. Supernatants were used to determine virus titers by hemaglutination assay. (B) Time-of-addition assay. After 2 h at 4 ºC to allow virus binding but not virus entry, the infected cells were treated with 20 nmol/L EGCG at the indicated times (immediately or at 2, 4, 6, 8, 10, 14, 18, 22, and 26 h post-adsorption) until supernatants and cell lysates were collected at 28 h post-infection. Total viral RNAs were extracted from cell lysates, reverse transcribed and assayed by qPCR as described in Materials and methods. Supernatants were used for hemagglutination assay. Data are presented as mean±SD of results from three independent experiments. (C) Cytoprotection effect of EGCG by time-of-addition assay. Light microscopy images were captured at an original magnification of ×200. VC indicates viral controls.