3. During the Gram staining of bacteria, iodine is used as:
(A) Primary stain
(B) Mordant
(C) Secondary stain
(D) Decolourising agent
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Answer:
mordant
Explanation:
Iodine is subsequently added as a mordant to form the crystal violet-iodine complex so that the dye cannot be removed easily. This step is commonly referred to as fixing the dye.
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