3. How would you test the presence of starch in leaves?
please give explanation step by step
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Answers
The presence of starch in leaves can be tested by Iodine test. When we remove chlorophyll from leaf by boiling it in alcohol and then put 2 drops of iodine solution, its colour change to blue indicates the presence of starch.
Answer:
Explanation:
Starch testing
Iodine solution is used to test leaves for the presence of starch. You need to:
1. heat a plant leaf in boiling water for 30 seconds (this stops its chemical reactions)
2. heat it in boiling ethanol for a few minutes (this removes most of its colour)
3. wash with water and spread onto a white tile
4.add iodine solution from a dropping pipette
After a few minutes, the parts of the leaf that contain starch turn blue-black.
Note that ethanol is heated using a hot water bath. Ethanol boils at 78°C, so a tube of it boils when placed in a beaker of hot water. This is safer than using a Bunsen burner because ethanol is flammable.