Question

3. In vitro amplification of DNA or RNA
segment is known as ........
a. chromatography
b. southern blotting
c. polymerase chain reaction
d. gel electrophoresis​

Answer 1

Answer: option c

polymerase chain reaction

Explanation:

Answer 2

The correct option is (C) Polymerase chain reaction.

Explanation:

• Kary Mullis invented PCR (Polymerase Chain Reaction) in the 1980s, and it was a game-changing approach.
• The ability of DNA polymerase to manufacture additional strands of DNA complementary to the template strand is used in PCR.
• Because DNA polymerase can only add a nucleotide to a 3'-OH group that already exists, it requires a primer to add the initial nucleotide.
• This criterion allows the researcher to define a specific area of the template sequence that he or she wants to amplify.
• The exact sequence will be amassed in billions of copies at the end of the PCR operation.