Question

3. Which filter are absorbed visible
radiation and transmit UV radiation ?
A. Primary filter
B. Secondary filter
C. A and B
D. All of the above​

Answer 1

Answer:

3)C)A and B

Explanation:

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Answer 2

Answer:

Option A. Primary filter is the correct answer.

Explanation:

These are nothing more than optical filters that work on the principle of absorbing unwanted light and transmitting light of desired wavelengths. Inexpensive fluorometers have primary and secondary filters.

Primary filter:

- Absorb visible light and transmit UV light.

Secondary filter:

- Absorbs UV rays and transmits visible light.

• This is an analytical method for detecting and measuring the fluorescence of the compounds used.
• Ultraviolet light stimulates the compound to emit visible light. energy/light
• Radiation emitted by matter has a longer wavelength than that absorbed. This process of emitting longer wavelength radiation than is absorbed is called luminescence.

Fluorescence is a type of luminescence caused by photons that excite molecules, leaving them in an electronically excited state. It is an optical phenomenon in which molecular absorption of energy in the form of photons causes emission of longer wavelength fluorescent photons.

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