Question

3. Which of the following is the basic
requirement of PCR reaction? *
O Two oligonucleotide primers
O DNA segment to be amplified
O A heat-stable DNA polymerase
O All of the above​

Answer 1

Answer:

a heat stable DNA polymerase

Answer 2

Answer:

All of the above

Explanation:

PCR stands for "Polymerase Chain Reaction".

It is a technique wherein a single DNA segment can be copied into several fragments. It is a popular tool in molecular biology used in medical science, forensic science, research and genetics.

PCR involves enzymatic replication of the DNA into millions of copies.

It involves the binding of two oligonucleotide primers to the DNA segment to be amplified and then a heat-stable DNA polymerase is used to replicate the DNA molecule.

The polymerase is used to synthesize the new strands of DNA.

Therefore, all three options two oligonucleotide primers, DNA segment to be amplified and a heat-stable DNA polymerase is required for the process of PCR.