35. The PCR variants used for the study of RNA expression level is called
(A) Anchored PCR
(B) Nested PCR
(C) RT PCR
(D) qPCR
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Answer:
(B) Nested PCR
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RT-PCR, also known as Reverse Transcriptase PCR, is a variation of the polymerase chain reaction that typically measures RNA expression levels. In RT-PCR, complementary DNA (cDNA) is made by reverse transcribing of the RNA templates with the enzyme reverse transciptase.
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