Question

4. A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA? a.) Sal I b.) Pvu I c.) EcoRI d.) Hind III


5. The most important cause of biodiversity loss is: a.) Over exploitation of economic species b.) Habitat loss and fragmentation c.) Invasive species d.) Breakdown of plant-pollinator relationships

Answer 1

4. EcoRI is a restriction site.

Explanation:

• EcoRI is a "restriction endonuclease". Restriction endonuclease is enzymes that are isolated from bacteria which is used to cut particular sequences in DNA.
• EcoRI also read as "eco R" is common and it is used to cut a specific sequence in a "plasmid".
• It is used to ligate. It's ligation results in the formation of the sticky end where there is a single strand of DNA overhanging.

5. Habitat loss and fragmentation is the major cause of biodiversity loss.

Answer: Option (b)

Explanation:

• Biodiversity is the total amount of biological organisms i.e. The total flora and fauna of a particular region. Biodiversity is nowadays getting reduced by various degrees all over across the globe.
• This is happening mainly due to the invasion of human civilization and artificial environment towards the forest areas.
• Their natural habitat is getting disrupted. Over-exploitation of the economic species is definitely one of the causes, but not the principal one.

Learn more about most Important cause of biodiversity loss and  biotechnologist:

The most important cause of biodiversity loss is:

a.)Over exploitation of economic species

b.)Habitat loss and fragmentation

c.)Invasive species

d.)Breakdown of plant-pollinator relationships

https://brainly.in/question/12768281

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?

a.)Sal I

b.)Pvu I

c.)EcoRI

d.)Hind III

https://brainly.in/question/12768083