Question

4. A mixture of lysine, alanine, isoleucine, arginine and glutamic acid are separated by ionic exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2?

3

a) With a cation exchange resin?

b) With an anion exchange resin?

c) Which column would give the best separation?​

Answer 1

Answer:

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Answer 2

4. A mixture of lysine, alanine, isoleucine, arginine and glutamic acid are separated by ionic exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2?

ans - The optimum pH to separate a mixture of lysine, arginine, and cysteine using electrophoresis (See the table in the image.) is at pH about 9.5