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Q.15 In a fed-batch culture operating with intermittent addition of glucose solution, values of
the following parameters are given at time t= 2 h, when the system is at quasi-steady
state.
V= 1000 ml
F=200 ml/h
H = 0,3 h-l
So= 100 g glucose/l
K=0.1 g glucose/
Y, M = 0.5 gdw cells/g glucose
X = 30 g
a. Find VO (the initial volume of the culture).
b. Determine the concentration of growth-limiting substrate in the vessel at quasi-
steady state.
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Answer:
a.find vo ( the initial volume
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