Question

4. Study of pollen germination on slide.sliod draw the diagrams. ​

Answer 1

Answer:

Apparatus and materials required:

Fresh seasonal flower slides,

microscope, beaker, cavity slide, boric acid, sucrose.

Procedure:

Prepare a nutrient solution by dissolving 10g sucrose and 10 mg boric acid in 100 ml water.

Take a few drops of this solution on a clear slide, dust a few pollen grains from the stamen of a mature flower in it.

Obverse the slide under the microscope after 5 minutes. Keep observing the slide regularly after intervals of half an hour.

Observations:

In a nutrient solution or nutrient-rich medium, the pollen grains germinate. The tube/vegetative cell enlarges and comes out the pollen grains throughone of the germ pores to form a pollen tube. The generative cell nucleus soon passes into it and dives to make 2 sperm nuclei (male gametes). Each male gamete is lenticular to spherical in outline.

Precautions:

Flowers should be freshly picked

Use a clear cavity slide to observe pollen grain

Answer 2

*Please refer to the picture for the diagram.

AIM - To study pollen germination on a slide.

MATERIAL AND APPARATUS- Flowers, Boric acid, beaker,

sucrose, compound microscope, and caviry slide.

PROCEDURE-

• Dissolve 10 grams of sucrose and 10 mg of boric acid in 100 ml of water to form a nutrient solution.
• Put few drops of this solution on cavity slide. Over this put few pollen grain by brushing gently from the pollen of a mature flower.
• After keeping the slide to set for 5 minutes observe it under compound microscope in 30 minutes time interval.

OBSERVATION-

• Pollen grains germinate in nutrient solution.
• The germination process is characterised by tje formation enlarged vegetative/tube cell.
• The nucleus of the generative cell makes two male gametes by the process of meiosis.

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