Question

5. Which of the followings is not required in next generation DNA sequencing
a. Massive parallel pyroseqencing
b. Cloning of the DNA fragment
c. Amplification of the fragment
d. Imaging based base calling

Answer 1

Explanation:

the amplification of dna is not required in the whole process of dna sequencing

Answer 2

Next-Generation Sequencing:

Here the amplification DNA is not required as the whole process is automated.