Question

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Explain how plant cell and animal cell are made competent for transformation with rDNA​

Answer 1

Answer:

Competent host is recombinant DNA technology is Agrobacterium cell. Competent host cell is required for transformation with recombinant DNA. Different types of available host cells are like E.cola, yeast, animal and plant cells. Eukaryotic cells will be preferred host, yeast-cells are preferred because this are simple eukaryotic organism and like bacteria are single-celles, easy to grow manipulated. Bacteria also acts enabled to pick up the recombinant-DNA.

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Answer 2

Answer:

Since DNA is hydrophillic it cannot pass through cell membrane hence bacterialcells are made competent by treatment with a specific concentration of a divalent cation such as Ca++ which increases efficiency of entry of DNA through the pores of cell wall b i plant cells - biolistic/ gene guns ii animal cells- Micro injection

Explanation:

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