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What to do here?
Make a temporary mount of fleshy leaves of onion to show the epidermal cells.
Answers
Explanation:
To prepare a stained, temporary mount of onion peel and to study its cells.
Theory
All living organisms are composed of cells. New cells arise by the division of pre-existing cells. Cell is the structural and functional unit of life. In plants, cells have an outermost rigid cell wall beneath which is a cell membrane. The cell membrane encloses cytoplasm, cell organelles, and a nucleus.
Materials Required
An onion bulb, slides, cover slips, two watch glasses, needle, brush, forceps, razor blade, compound microscope, blotting paper, methylene blue solution (or safranin), glycerine, and water.
Procedure
Step 1: One fleshy scale leaf of an onion is taken. It is broken into two and a thin membranous peel is pulled out using a forcep adhering to the inner surface of the leaf. This is the epidermal peel.
Step 2: The peel is placed in a watch glass containing water and is cut into small rectangular pieces.
Step 3: 1 or 2 drops of methylene blue or safrarin is mixed in a small quantity of water taken in another watch glass. The peel is transfered into it. Peels are leave for about 3 minutes. The peel is dipped in water to remove excess stain.
Step 4: Clean slide is taken with a drop of glycerine in the middle and a brush is used to transfer the washed and stained peel on to it.
Step 5: Cover slip is placed over it by slowly lowering it with a needle. Avoid entry of air bubbles.
Step 6: Excess glycerine is removed from the edges of cover slip with the help of a piece of blotting paper.
Step 7: The slide is observed under the microscope, first in low power and then in high power.
Step 8: A labelled diagram of the cells is drawn as seen under microscope.
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