6. How can you prove that starch is present in leaves?
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The presence of starch in leaves can be tested by the Iodine test. When we remove chlorophyll from the leaf by boiling it in alcohol and then put two drops of iodine solution, it is colour change to blue indicates the presence of starch.
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Iodine solution is used to test leaves for the presence of starch.
You need to:
- heat a plant leaf in boiling water for 30 seconds
- add the leaf to boiling ethanol in a water bath for a few minutes
- wash with water to rehydrate and soften the leaf and spread onto a white tile.
- add iodine solution from a dropping pipette
After a few minutes, the parts of the leaf that contain starch turn the iodine from brown to blue/black.
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