Question

♡7) Define Exclusion limit.”​

Answer 1

EXCLUSION LIMIT : In SEC, this is the upper limit of molecular weight (or size), beyond which molecules will elute at the same retention volume. Many SEC packings are referred to by their exclusion limit. For example, a 105 column of porous silica gel will exclude any compounds with a molecular weight higher than 100,000, based on a polystyrene calibration standard.