Question

7. Which of the following is responsible for the initiation of RNA polymerase activity?
a) Promoter region
b) Initiation site
c) Sigma factor
d) rho factor​

Answer 1

Answer:

Its sigma factor

Explanation:

RNA polymerase binding in bacteria involves the sigma factor recognizing the core promoter region containing the −35 and −10 elements (located before the beginning of sequence to be transcribed) and also, at some promoters, the α subunit C-terminal domain recognizing promoter upstream elements.

Answer 2

Sigma factor is responsible for the initiation of RNA polymerase activity.

Explanation:

Promoter region: The promoter is a DNA region where gene transcription is initiated. Promoters are a vital component of expression vectors because they control the DNA binding of RNA polymerase.

Initiation site: The DNA site from which the first RNA nucleotide is transcribed is called the + 1 site or the initiation site. Nucleotides that come before the initiation site are given negative numbers and are said to be upstream.

Sigma factor: A sigma factor (Δ factor) (specificity factor) is a protein that is needed to initiate transcription in bacteria. It is a bacterial transcription initiation factor that allows the specific binding of RNA polymerase (RNAP) to gene promoters.

Rho factor: Rho factor (Rho factor) is a prokaryotic protein involved in the termination of transcription.

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