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Is mass of co2....
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in simultaneous measurements of CO2 uptake
and O2 evolution in a sunflower leaf, O2 evolution changed by
7% more than CO2 uptake when light intensity was varied.
Since the O2/CO2 exchange ratio is 1, small differences are
important. Thus, these gas exchange measurements need
precise calibration. In this work, we describe a new
calibration procedure for such simultaneous measurements,
based on the changes of O2 concentration caused by the
addition of pure CO2 or O2 into a flow of dry air (20.95% O2)
through one and the same capillary. The relative decrease in
O2 concentration during the addition of CO2 and the relative
increase in O2 concentration during the addition of O2
allowed us to calibrate the CO2 and O2 scales of the
measurement system with an error (relative standard
deviation, RSD) of 51%. Measurements on a sunflower leaf
resulted in an O2/CO2 ratio between 1.0 and 1.03
under different CO2 concentrations and light intensities,
in the presence of an ambient O2 concentration of
20–50 mmol mol-
1
. This shows that the percentage use of
reductive power from photochemistry in synthesis of inorganic
or organic matter other than CO2 assimilation in the C3 cycle
is very low in mature leaves and, correspondingly,
the reduction of alternative acceptors is a weak source of
coupled ATP synthesis.
Keywords: Alternative electron transport — Carbon dioxide
— Oxygen — Photosynthesis.