9. In dark field microscope, for good visibility
type of object slides and coverslips are
used instead of glass,
(1 Point)
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Answer:
The best specimens for dark field are those that have refractive objects scattered about with empty space between them. No dark field occurs if objects are too crowded or if a thick solid specimen turns light into the microscope.
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Explanation:
Dark field optics are a low cost alternative to phase contrast optics. The contrast and resolution obtained with inexpensive dark field equipment may be superior to what you have with student grade phase contrast equipment. It is surprising that few manufacturers and vendors promote the use of dark field optics.
A dilute suspension of yeast cells makes a good practice specimen for dark field optics, particularly when cultured with living Paramecium.
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